Elizabeth Henstridge
as Jemma Simmons
Chloe Bennet
as Daisy "Skye" Johnson / Quake
Ming-Na Wen
as Melinda May
Clark Gregg
as Phil Coulson
Iain De Caestecker
as Leo Fitz
Henry Simmons
as Alphonso "Mack" Mackenzie
Natalia Cordova-Buckley
as Elena "Yo-Yo" Rodriguez
Brett Dalton
as Grant Ward
Jeff Ward
as Deke Shaw
Nick Blood
as Lance Hunter
Adrianne Palicki
as Bobbi Morse / Mockingbird
Luke Mitchell
as Lincoln Campbell
John Hannah
as Holden Radcliffe
Clark Gregg
as Sarge